streptococcus pneumoniae atcc 49619  (ATCC)

Journal: iScience

Article Title: Persistent viral control status is associated with enhanced innate immune responses in people with HIV-1

doi: 10.1016/j.isci.2026.114807

Figure Lengend Snippet: Monocytes and lymphocytes' responses to stimulation (A and B) PBMCs from HIV controllers ( n = 95), consisting of elite controllers (ECs, n = 20), viremic controllers (VCs, n = 28) [persistent HIV controllers], transient controllers (TCs, n = 43) and normal progressor on ART ( n = 1317) were stimulated with the TLR ligands Poly:IC, IMQ, LPS, the peptides HIV ENV pool and pp65 CMV, S. pneumoniae and IL-1α for 24 h and cytokines (IL-10, IL-1β, IL1-Ra, IL-6, IL-8, TNF) and chemokines (MCP-1, MIP-1α) were measured by ELISA. (C) Log2-transformed concentration of MCP-1, IL-1β, IL-10, IL-6, and IL-8 upon stimulation across the different groups of PLHIV. (D) PBMCs from the same groups of individuals were stimulated for 7 days with C. albicans conidia, C. albicans hyphae, E. coli , M. tuberculosis, PHA, S. aureus , S. pneumoniae, and cytokines (IFNγ, IL-10, IL-17, IL-22, and IL-5) were measured by ELISA. (E) Log2-transformed concentration of IL-10, IFNγ, and IL-22 upon stimulation across the different groups of PLHIV. Rank-based regression with age, sex, seasonality, and ancestry as confounders was used for p -value calculation, ∗ nominal p < 0.05. Heatmaps represent the estimate from rfit divided by the mean cytokine level (scaled beta-values). The data are represented in boxplots showing the median (horizontal line) with standard errors.

Article Snippet: Streptococcus pneumoniae , ATCC , ATCC 49619.

Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay, Concentration Assay

Journal: iScience

Article Title: Persistent viral control status is associated with enhanced innate immune responses in people with HIV-1

doi: 10.1016/j.isci.2026.114807

Figure Lengend Snippet: Functional examination of monocytes and lymphocytes in first-degree family members of persistent controllers and non-controllers (A) PBMCs from first-degree family members of persistent controllers ( n = 19) and non-controllers ( n = 21) matched by age, sex, and ancestry were stimulated with the TLR ligands Poly:IC, IMQ, LPS, the peptides HIV ENV pool and pp65 CMV, S. pneumoniae, and rhIL-1α for 24 h, and cytokines (IL-10, IL-1β, IL1-Ra, IL-6, IL-8, TNF) and chemokines (MCP-1, MIP-1α) were measured by ELISA. (B) PBMCs from the same group of individuals were stimulated for 7 days with C. albicans conidia, C. albicans hyphae, E. coli , M. tuberculosis, PHA, S. aureus , S. pneumoniae, and the cytokines (IFNγ, IL-10, IL-17, IL-22, IL-5) were measured by ELISA. Heatmaps represent the estimate divided by the mean cytokine level (scaled concentration). Statistical significance was assessed by the Wilcoxon rank-sum test. (C) Log2 TNF, IL-1β, and MIP-1α concentration upon stimulation across the different groups of family members. (D) Log2 IFNγ and IL-10 concentration upon stimulation across the different groups of family members. (E) Adherent monocytes from the family of persistent controllers and family of non-controllers were exposed to C. albicans β-glucan for 24 h and restimulated with LPS at day 7. Cytokines (TNF, IL-6, and IL-1Ra) concentrations were measured by ELISA. (F) Fold increase in TNF, IL-6, and IL-1Ra production upon β-glucan training normalized to RPMI. Comparisons between trained monocytes and RPMI control; Comparisons between family members of persistent controllers vs. family of non-controllers, before and after training. For the determination of statistical significance Wilcoxon rank-sum test was performed ( p < 0.05). The data are represented as boxplots showing the median with standard errors.

Article Snippet: Streptococcus pneumoniae , ATCC , ATCC 49619.

Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Control